Río Negro or Rio Negro ("black river" in, respectively, Spanish and Portuguese) may refer to:

Political divisions

Río Negro Province
Río Negro Department
Río Negro, Chile, municipality in Osorno Province, Los Lagos Region
Rionegro, Antioquia a town and municipality
Rionegro, Santander a town and municipality
Rionegro Province a Province of Cundinamarca Department
Río Negro Municipality
Río Negro, Junín, a town in Satipo Province, Junín Region Sports

Atlético Rio Negro Clube, a Brazilian football team from Manaus, Amazonas state
Atlético Rio Negro Clube (RR), a Brazilian football team from Boa Vista, Roraima